Hotspot

trnS-GCU-trnR-UCU

LSC · 33296-34724 · 1429 bp

Back to Desmidorchis

Pi 0.00071

MarkerSeek score 60.50

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1429
variable_sites: 1
pi: 0.00071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1480-1500 bp	1.0	64.7
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1479-1499 bp	1.0	64.7
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1486-1506 bp	1.0	64.0
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1485-1505 bp	1.0	64.0
trnS-GCU-trnR-UCU_p5	AGCAATCCGACGCTTTAGTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	1482-1502 bp	1.0	61.8