Hotspot

rps16-trnQ-UUG

LSC · 30819-32109 · 1291 bp

Back to Desmidorchis

Pi 0.00157

MarkerSeek score 61.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1291
variable_sites: 2
pi: 0.00157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAATGGATCATGGCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1372-1385 bp	1.0	78.9
rps16-trnQ-UUG_p2	TGGATCATGGCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1366-1379 bp	1.0	77.2
rps16-trnQ-UUG_p3	CCACAATGGATCATGGCCTT	TAAACTAAATGGGGCGTGGC	50.0	50.0	58.2	58.5	1429-1442 bp	1.0	75.2
rps16-trnQ-UUG_p4	GGATCATGGCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.7	58.1	1365-1378 bp	1.0	75.0
rps16-trnQ-UUG_p5	CCACAATGGATCATGGCCTT	TTCGGAGGTTCGAATCCTTC	50.0	50.0	58.2	57.3	1376-1389 bp	1.0	74.2