Hotspot

trnK-UUU-rps16

LSC · 28918-29723 · 806 bp

Back to Desmidorchis

Pi 0
MarkerSeek score 60.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
806
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TCAAAAGGGTGGGGGTTTTT 55.0 45.0 57.9 57.7 944-957 bp 1.0 78.4
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTCAAAAGGGTGGGGGTTTT 55.0 45.0 57.9 57.7 945-958 bp 1.0 78.4
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC TTTCAAAAGGGTGGGGGTTT 55.0 45.0 57.9 57.7 946-959 bp 1.0 78.4
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TTTTCAAAAGGGTGGGGGTT 55.0 45.0 57.9 57.7 947-960 bp 1.0 78.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCAAAAGGGTGGGGGTTTTT 50.0 45.0 58.2 57.7 882-895 bp 1.0 77.7