primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 20 20 55.0 55.0 58.1 57.9 0.0 0.0 0.203648 24712 25039 398-398 1.000000 78.8 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.208010 24712 25039 412-412 1.000000 78.7 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.209518 24712 25039 411-411 1.000000 78.7 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.209518 24712 25039 413-413 1.000000 78.7 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 20 20 55.0 55.0 58.1 58.3 0.0 0.0 0.465225 24712 25039 447-447 1.000000 76.6 trnK-UUU_p1 trnK-UUU 1 TGCTAGTGTCAACTAATATCGA CGATTGTATTTATGCACTTCT 22 21 36.4 33.3 54.5 52.1 0.0 0.0 12.422332 26322 28917 2808-2820 1.000000 40.5 trnK-UUU_p2 trnK-UUU 2 TGCTAGTGTCAACTAATATCGATCT CGATTGTATTTATGCACTTCT 25 21 36.0 33.3 57.5 52.1 0.0 0.0 12.444925 26322 28917 2808-2820 1.000000 40.5 trnK-UUU_p3 trnK-UUU 3 TGCTAGTGTCAACTAATATCGA CGATTGTATTTATGCACTTCTT 22 22 36.4 31.8 54.5 53.0 0.0 0.0 12.521137 26322 28917 2808-2820 1.000000 40.5 trnK-UUU_p4 trnK-UUU 4 TGCTAGTGTCAACTAATATCGATCT CGATTGTATTTATGCACTTCTT 25 22 36.0 31.8 57.5 53.0 0.0 0.0 12.543730 26322 28917 2808-2820 1.000000 40.5 trnK-UUU_p5 trnK-UUU 5 TGCTAGTGTCAACTAATATCGA CGATTGTATTTATGCACTTCTTT 22 23 36.4 30.4 54.5 53.8 0.0 0.0 12.696631 26322 28917 2808-2820 1.000000 40.5 matK_p1 matK 1 TGTTACACCAACATTTCAGA AAGTTTACCCGTTTCCGAGG 20 20 35.0 50.0 52.6 57.8 0.0 0.0 5.539720 26616 28172 1760-1760 1.000000 40.2 matK_p2 matK 2 TGTTACACCAACATTTCAGA TTTGCTAACCCCAAAACCGT 20 20 35.0 45.0 52.6 58.2 0.0 0.0 5.587416 26616 28172 1689-1689 1.000000 40.2 matK_p3 matK 3 TGTTACACCAACATTTCAGAA AAGTTTACCCGTTTCCGAGG 21 20 33.3 50.0 53.6 57.8 0.0 0.0 5.605337 26616 28172 1760-1760 1.000000 40.2 matK_p4 matK 4 TGTTACACCAACATTTCAGAA TTTGCTAACCCCAAAACCGT 21 20 33.3 45.0 53.6 58.2 0.0 0.0 5.653033 26616 28172 1689-1689 1.000000 40.2 matK_p5 matK 5 TGTTACACCAACATTTCAGA ACCCGTTTCCGAGGTATCTA 20 20 35.0 50.0 52.6 57.6 0.0 0.0 5.807593 26616 28172 1754-1754 1.000000 40.2 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCGATGGTATGGACGAATCC TCAAAAGGGTGGGGGTTTTT 20 20 55.0 45.0 57.9 57.7 0.0 0.0 0.352360 28918 29723 944-957 1.000000 78.4 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CCGATGGTATGGACGAATCC TTCAAAAGGGTGGGGGTTTT 20 20 55.0 45.0 57.9 57.7 0.0 0.0 0.352360 28918 29723 945-958 1.000000 78.4 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CCGATGGTATGGACGAATCC TTTCAAAAGGGTGGGGGTTT 20 20 55.0 45.0 57.9 57.7 0.0 0.0 0.352360 28918 29723 946-959 1.000000 78.4 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CCGATGGTATGGACGAATCC TTTTCAAAAGGGTGGGGGTT 20 20 55.0 45.0 57.9 57.7 0.0 0.0 0.352360 28918 29723 947-960 1.000000 78.4 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 AAGCCGAGTACTCTACCGTT TCAAAAGGGTGGGGGTTTTT 20 20 50.0 45.0 58.2 57.7 20.3 0.0 0.440494 28918 29723 882-895 1.000000 77.7 rps16_p1 rps16 1 ACGGGAAAAGGAAAAAGGGG CTCCGACGTCTACAAAGCAA 20 20 50.0 50.0 58.3 57.9 0.0 14.7 0.348270 29724 30818 1268-1268 1.000000 77.4 rps16_p2 rps16 2 ATATACAAGCAACTGCCCCC CTCCGACGTCTACAAAGCAA 20 20 50.0 50.0 57.6 57.9 0.0 14.7 0.425298 29724 30818 1217-1218 1.000000 76.8 rps16_p3 rps16 3 ACGGGAAAAGGAAAAAGGGG GACTCCGACGTCTACAAAGC 20 20 50.0 55.0 58.3 58.4 0.0 14.7 0.672442 29724 30818 1270-1270 1.000000 74.8 rps16_p4 rps16 4 ATATACAAGCAACTGCCCCC GACTCCGACGTCTACAAAGC 20 20 50.0 55.0 57.6 58.4 0.0 14.7 0.749471 29724 30818 1219-1220 1.000000 74.2 rps16_p5 rps16 5 ACGAGTAGAACGGGAAAAGG CTCCGACGTCTACAAAGCAA 20 20 50.0 50.0 57.3 57.9 0.0 14.7 0.798339 29724 30818 1277-1277 1.000000 73.8 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 CCACAATGGATCATGGCCTT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 3.3 16.9 0.291743 30819 32109 1372-1385 1.000000 78.9 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 TGGATCATGGCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.6 58.1 3.3 16.9 0.501244 30819 32109 1366-1379 1.000000 77.2 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CCACAATGGATCATGGCCTT TAAACTAAATGGGGCGTGGC 20 20 50.0 50.0 58.2 58.5 3.3 0.0 0.749847 30819 32109 1429-1442 1.000000 75.2 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 GGATCATGGCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 20 20 55.0 55.0 58.7 58.1 3.3 16.9 0.772792 30819 32109 1365-1378 1.000000 75.0 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 CCACAATGGATCATGGCCTT TTCGGAGGTTCGAATCCTTC 20 20 50.0 50.0 58.2 57.3 3.3 16.9 0.883115 30819 32109 1376-1389 1.000000 74.2 trnS-GCU-trnR-UCU_p1 trnS-GCU-trnR-UCU 1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.0 56.9 0.0 0.0 2.056187 33296 34724 1480-1500 1.000000 64.7 trnS-GCU-trnR-UCU_p2 trnS-GCU-trnR-UCU 2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.0 56.9 0.0 0.0 2.057575 33296 34724 1479-1499 1.000000 64.7 trnS-GCU-trnR-UCU_p3 trnS-GCU-trnR-UCU 3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 20 22 50.0 45.5 58.0 57.9 0.0 0.0 2.139515 33296 34724 1486-1506 1.000000 64.0 trnS-GCU-trnR-UCU_p4 trnS-GCU-trnR-UCU 4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 20 22 50.0 45.5 58.0 57.9 0.0 0.0 2.140903 33296 34724 1485-1505 1.000000 64.0 trnS-GCU-trnR-UCU_p5 trnS-GCU-trnR-UCU 5 AGCAATCCGACGCTTTAGTC AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.4 56.9 0.0 0.0 2.410028 33296 34724 1482-1502 1.000000 61.8 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 ATTCCCTCATTTGCATCCCC CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.174726 51767 53001 1323-1323 1.000000 78.7 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 GCAAACCCCGATCAATGAAC CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 57.7 58.1 0.0 0.0 0.360154 51767 53001 1391-1391 1.000000 77.2 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 ATTCCCTCATTTGCATCCCC AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 57.9 58.4 0.0 14.6 0.448089 51767 53001 1305-1305 1.000000 76.5 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 GCAAACCCCGATCAATGAAC AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 57.7 58.4 0.0 14.6 0.633517 51767 53001 1373-1373 1.000000 75.0 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 ATTCCCTCATTTGCATCCCC TTTGTTGATCAGGCGACACC 20 20 50.0 50.0 57.9 58.8 0.0 0.0 0.857777 51767 53001 1342-1342 1.000000 73.3 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 AGAGATGTCCTGAACCGCTA ACCGGAGTTTTATGGAAGCC 20 20 50.0 50.0 57.9 57.9 0.0 9.5 0.269674 56422 57444 1143-1143 1.000000 77.9 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 AGATGTCCTGAACCGCTAGA ACCGGAGTTTTATGGAAGCC 20 20 50.0 50.0 57.9 57.9 0.0 9.5 0.269674 56422 57444 1141-1141 1.000000 77.9 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 CCTTTCCTAGTCCCCTACCC ACCGGAGTTTTATGGAAGCC 20 20 60.0 50.0 58.2 57.9 0.0 9.5 0.320411 56422 57444 1199-1199 1.000000 77.5 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 CTTTCCTAGTCCCCTACCCC ACCGGAGTTTTATGGAAGCC 20 20 60.0 50.0 58.2 57.9 0.0 9.5 0.320411 56422 57444 1198-1198 1.000000 77.5 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 GATGTCCTGAACCGCTAGAC ACCGGAGTTTTATGGAAGCC 20 20 55.0 50.0 57.8 57.9 0.0 9.5 0.335630 56422 57444 1140-1140 1.000000 77.4 psbC_p1 psbC 1 TCTCAGGAAATTCGAGCAGC AGAATGACCCGATCCCAAGA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.136161 59756 61177 1609-1609 1.000000 79.0 psbC_p2 psbC 2 AAATTCGAGCAGCGGAAGAT AGAATGACCCGATCCCAAGA 20 20 45.0 50.0 58.0 58.1 7.8 0.0 0.150263 59756 61177 1602-1602 1.000000 78.8 psbC_p3 psbC 3 TCTCAGGAAATTCGAGCAGC TGACCCGATCCCAAGATGTA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.184194 59756 61177 1605-1605 1.000000 78.6 psbC_p4 psbC 4 AAATTCGAGCAGCGGAAGAT TGACCCGATCCCAAGATGTA 20 20 45.0 50.0 58.0 57.8 7.8 0.0 0.198297 59756 61177 1598-1598 1.000000 78.5 psbC_p5 psbC 5 AGGTATTCGTGCTTGGATGG AGAATGACCCGATCCCAAGA 20 20 50.0 50.0 57.7 58.1 0.0 0.0 0.452488 59756 61177 1538-1538 1.000000 76.4 psbZ-trnG-GCC_p1 psbZ-trnG-GCC 1 GTGCCCGTTGTATTTGCTTC GGGTAGAGCAGTTTGGTAGC 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.048400 62021 62352 768-782 1.000000 81.2 psbZ-trnG-GCC_p2 psbZ-trnG-GCC 2 GTGCCCGTTGTATTTGCTTC TTCATCGACGTTGTTTTGCG 20 20 50.0 45.0 58.0 58.0 0.0 2.0 0.034744 62021 62352 1074-1088 1.000000 80.8 psbZ-trnG-GCC_p3 psbZ-trnG-GCC 3 ATTAGCGTGCCCGTTGTATT TTCATCGACGTTGTTTTGCG 20 20 45.0 45.0 58.0 58.0 0.0 2.0 0.044612 62021 62352 1080-1094 1.000000 80.7 psbZ-trnG-GCC_p4 psbZ-trnG-GCC 4 CAACCACTCAGCCATCTCTC GGGTAGAGCAGTTTGGTAGC 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.046944 62021 62352 1174-1188 1.000000 80.7 psbZ-trnG-GCC_p5 psbZ-trnG-GCC 5 CAACCACTCAGCCATCTCTC TTCATCGACGTTGTTTTGCG 20 20 55.0 45.0 58.0 58.0 0.0 2.0 0.033287 62021 62352 1480-1494 1.000000 80.6 trnG-GCC_p1 trnG-GCC 1 AGGAGAGTCTTTGGTCTGGG ACTACACGATCACCGACAAC 20 20 55.0 50.0 58.3 57.7 0.0 0.0 0.692382 62353 62423 263-265 1.000000 75.4 trnG-GCC_p2 trnG-GCC 2 GGAGAGTCTTTGGTCTGGGA ACTACACGATCACCGACAAC 20 20 55.0 50.0 58.4 57.7 0.0 0.0 0.697200 62353 62423 262-264 1.000000 75.3 trnG-GCC_p3 trnG-GCC 3 AGAGTCTTTGGTCTGGGACT ACTACACGATCACCGACAAC 20 20 50.0 50.0 57.6 57.7 0.0 0.0 0.750250 62353 62423 260-262 1.000000 74.9 trnG-GCC_p4 trnG-GCC 4 AGGAGAGTCTTTGGTCTGGG GGGAGGCTATAGGAAAGGGA 20 20 55.0 55.0 58.3 57.6 0.0 1.0 0.767190 62353 62423 301-303 1.000000 74.7 trnG-GCC_p5 trnG-GCC 5 GGAGAGTCTTTGGTCTGGGA GGGAGGCTATAGGAAAGGGA 20 20 55.0 55.0 58.4 57.6 0.0 1.0 0.772009 62353 62423 300-302 1.000000 74.6 psaA-ycf3_p1 psaA-ycf3 1 TGAGAAATGACCCGGTTTGG ATCAAGCTGCCGAGTATTGG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.136672 67777 68746 1167-1167 1.000000 79.0 psaA-ycf3_p2 psaA-ycf3 2 GCCCATTCCTCGAAAGAAGT ATCAAGCTGCCGAGTATTGG 20 20 50.0 50.0 57.9 58.0 4.1 0.0 0.156301 67777 68746 1148-1148 1.000000 78.8 psaA-ycf3_p3 psaA-ycf3 3 TGAGAAATGACCCGGTTTGG CGATCAAGCTGCCGAGTATT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.237622 67777 68746 1169-1169 1.000000 78.2 psaA-ycf3_p4 psaA-ycf3 4 GCCCATTCCTCGAAAGAAGT CGATCAAGCTGCCGAGTATT 20 20 50.0 50.0 57.9 58.1 4.1 0.0 0.257251 67777 68746 1150-1150 1.000000 78.0 psaA-ycf3_p5 psaA-ycf3 5 TGAGAAATGACCCGGTTTGG TTGAAGATCACGAGGCGTTT 20 20 50.0 45.0 58.1 57.8 0.0 0.0 0.259531 67777 68746 1096-1096 1.000000 78.0 atpB-rbcL_p1 atpB-rbcL 1 CTACATCTAGTACCGGGCCA AACACCGGCTTTGAATCCAA 20 20 55.0 45.0 58.0 58.3 6.3 0.0 0.310373 81209 81986 917-929 1.000000 78.6 atpB-rbcL_p2 atpB-rbcL 2 GCGCAAAGGCTACATCTAGT AACACCGGCTTTGAATCCAA 20 20 50.0 45.0 58.1 58.3 0.3 0.0 0.360169 81209 81986 926-938 1.000000 78.2 atpB-rbcL_p3 atpB-rbcL 3 GGAAACCCCAGAACCAGAAG AACACCGGCTTTGAATCCAA 20 20 55.0 45.0 58.1 58.3 0.0 0.0 0.390437 81209 81986 856-868 1.000000 78.1 atpB-rbcL_p4 atpB-rbcL 4 CGATACGTCCCAGGTTGTTT AACACCGGCTTTGAATCCAA 20 20 50.0 45.0 57.9 58.3 0.0 0.0 0.390444 81209 81986 887-899 1.000000 78.0 atpB-rbcL_p5 atpB-rbcL 5 TCTAGTACCGGGCCAATGAT AACACCGGCTTTGAATCCAA 20 20 50.0 45.0 57.9 58.3 6.3 0.0 0.398604 81209 81986 912-924 1.000000 77.9 rbcL-accD_p1 rbcL-accD 1 GAGGCTAGCAAATGGAGTCC GCCATAGCGATAGACAGAGC 20 20 55.0 55.0 58.0 58.0 18.2 0.0 0.050153 83415 83929 684-724 1.000000 83.7 rbcL-accD_p2 rbcL-accD 2 GAGGCTAGCAAATGGAGTCC AACCATTGAGTCCTCCTCCA 20 20 55.0 50.0 58.0 58.0 18.2 0.0 0.084376 83415 83929 613-653 1.000000 83.5 rbcL-accD_p3 rbcL-accD 3 GCTGCTTGTGAGGTATGGAA GCCATAGCGATAGACAGAGC 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.124430 83415 83929 654-694 1.000000 83.1 rbcL-accD_p4 rbcL-accD 4 GCTGCTTGTGAGGTATGGAA AACCATTGAGTCCTCCTCCA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.158653 83415 83929 583-623 1.000000 82.9 rbcL-accD_p5 rbcL-accD 5 AAATGGAGTCCTGAACTGGC GCCATAGCGATAGACAGAGC 20 20 50.0 55.0 57.8 58.0 11.3 0.0 0.217146 83415 83929 675-715 1.000000 82.4 psbE-petL_p1 psbE-petL 1 TGACAAATAACCAACCCGCA AAGCCGCTAGTAGAAAACCA 20 20 45.0 45.0 58.0 56.9 0.0 0.0 1.160799 90967 92101 1293-1293 1.000000 70.8 psbE-petL_p2 psbE-petL 2 TGACAAATAACCAACCCGCA AGCCGCTAGTAGAAAACCAA 20 20 45.0 45.0 58.0 56.9 0.0 0.0 1.160799 90967 92101 1292-1292 1.000000 70.8 psbE-petL_p3 psbE-petL 3 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCA 20 20 50.0 45.0 57.9 56.9 6.4 0.0 1.194794 90967 92101 1282-1282 1.000000 70.5 psbE-petL_p4 psbE-petL 4 CAACCCGCAATGAATAGGGA AGCCGCTAGTAGAAAACCAA 20 20 50.0 45.0 57.9 56.9 6.4 0.0 1.194794 90967 92101 1281-1281 1.000000 70.5 psbE-petL_p5 psbE-petL 5 AGAACGTTCTCCTGTGCTTC AGCCGCTAGTAGAAAACCAA 20 20 50.0 45.0 57.8 56.9 24.9 0.0 1.299803 90967 92101 1204-1204 1.000000 69.7 petD_p1 petD 1 GGGAGTTGAGGGGAATTGTC TCCCTAGATACACACGTCGT 20 20 55.0 50.0 57.9 57.6 0.0 0.0 0.538543 102922 104254 1352-1407 1.000000 78.9 petD_p2 petD 2 AGGGAGTTGAGGGGAATTGT TCCCTAGATACACACGTCGT 20 20 50.0 50.0 58.2 57.6 0.0 0.0 0.647866 102922 104254 1353-1408 1.000000 78.0 petD_p3 petD 3 GGGAGTTGAGGGGAATTGTC TTCCCTAGATACACACGTCGT 20 21 55.0 47.6 57.9 58.3 0.0 0.0 1.415226 102922 104254 1353-1408 1.000000 71.9 petD_p4 petD 4 TAGGGAGTTGAGGGGAATTGT TCCCTAGATACACACGTCGT 21 20 47.6 50.0 58.1 57.6 0.0 0.0 1.478970 102922 104254 1354-1409 1.000000 71.4 petD_p5 petD 5 AGGGAGTTGAGGGGAATTGT TTCCCTAGATACACACGTCGT 20 21 50.0 47.6 58.2 58.3 0.0 0.0 1.524549 102922 104254 1354-1409 1.000000 71.0 ycf1_p1 ycf1 1 TTCTTCGGTGGATCCCTCTT AGTCTGGATACGGCAAAATCA 20 21 50.0 42.9 58.0 57.4 9.9 0.0 1.674773 160942 161061 301-301 1.000000 66.9 ycf1_p2 ycf1 2 TTCTTCGGTGGATCCCTCTT CATGAAATGGCTTGGATTGGT 20 21 50.0 42.9 58.0 57.1 9.9 0.0 1.946431 160942 161061 325-325 1.000000 64.7 ycf1_p3 ycf1 3 CTTCTTCGGTGGATCCCTCT AGTCTGGATACGGCAAAATCA 20 21 55.0 42.9 58.5 57.4 9.9 0.0 2.136821 160942 161061 302-302 1.000000 63.2 ycf1_p4 ycf1 4 TTCTTCGGTGGATCCCTCTT AGTCTGGATACGGCAAAATCAT 20 22 50.0 40.9 58.0 57.8 9.9 0.0 2.203834 160942 161061 301-301 1.000000 62.6 ycf1_p5 ycf1 5 TTCTTCGGTGGATCCCTCTT GTCTGGATACGGCAAAATCA 20 20 50.0 45.0 58.0 55.8 9.9 0.0 2.212067 160942 161061 300-300 1.000000 62.6