Hotspot

accD

LSC · 56576-57793 · 1218 bp

Back to Descurainia

Pi 0.055

MarkerSeek score 73.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1218
variable_sites: 67
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCATCCTGCATGTTGTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	2233-2495 bp	1.0	80.7
accD_p2	GGCTTGAACCAAGTATCCCC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.2	57.9	2264-2526 bp	1.0	80.1
accD_p3	GGGCTTGAACCAAGTATCCC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.2	57.9	2265-2527 bp	1.0	80.1
accD_p4	CCATCCTGCATGTTGTCCTT	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.2	58.2	2215-2477 bp	1.0	79.8
accD_p5	CCATCCTGCATGTTGTCCTT	TGCAATTGCCGGAAAGACTA	50.0	45.0	58.2	57.8	2223 bp	0.5	57.2