Hotspot

rpl32-trnL-CUA

SSC · 112184-113066 · 883 bp

Back to Descurainia

Pi 0.0348

MarkerSeek score 58.30

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 883
variable_sites: 15
pi: 0.0348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.49
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGGGACAATAAAAAGCACGC	TGCAGACTGAGGCATTTTGT	50.0	45.0	58.0	58.0	2142 bp	0.5	59.8
rpl32-trnL-CUA_p2	GGGGACAATAAAAAGCACGC	TCGAACCGAGATGCTTTAGC	50.0	50.0	58.0	58.1	1632 bp	0.5	59.3
rpl32-trnL-CUA_p3	GGGGACAATAAAAAGCACGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1606 bp	0.5	58.9
rpl32-trnL-CUA_p4	GGGGACAATAAAAAGCACGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	1599 bp	0.5	58.4
rpl32-trnL-CUA_p5	GGGGACAATAAAAAGCACGC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	1604 bp	0.5	58.4