Hotspot

rpl16-rps3

LSC · 81499-81672 · 174 bp

Back to Descurainia

Pi 0.0482

MarkerSeek score 71.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 8
pi: 0.0482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	AAAATTTGGATATTCGTAGACG	45.0	31.8	57.1	52.7	225-229 bp	1.0	45.6
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	AAAAATTTGGATATTCGTAGACG	45.0	30.4	57.1	53.6	226-230 bp	1.0	45.6
rpl16-rps3_p3	ACCAACGAGTCACACACTAAG	AAAATTTGGATATTCGTAGACG	47.6	31.8	57.9	52.7	228-232 bp	1.0	45.6
rpl16-rps3_p4	AAACCAACGAGTCACACACT	AAAATTTGGATATTCGTAGACG	45.0	31.8	57.6	52.7	230-234 bp	1.0	45.5
rpl16-rps3_p5	AAACCAACGAGTCACACACT	AAAAATTTGGATATTCGTAGACG	45.0	30.4	57.6	53.6	231-235 bp	1.0	45.5