Hotspot

psbM-trnD-GAC

LSC · 28675-29703 · 1029 bp

Back to Descurainia

Pi 0.0189

MarkerSeek score 59.30

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 19
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	TGCAAGAATATTGACTTCCA	AATTGGTTAGAGCACCGCC	35.0	52.6	52.5	58.1	1089-1111 bp	1.0	43.1
psbM-trnD-GAC_p2	TGCAAGAATATTGACTTCCA	TCAATTGGTTAGAGCACCGC	35.0	50.0	52.5	58.6	1091-1113 bp	1.0	43.0
psbM-trnD-GAC_p3	ATGCAAGAATATTGACTTCCA	TCAATTGGTTAGAGCACCGC	33.3	50.0	53.2	58.6	1092-1114 bp	1.0	43.0
psbM-trnD-GAC_p4	TGCAAGAATATTGACTTCCAT	TCAATTGGTTAGAGCACCGC	33.3	50.0	53.2	58.6	1091-1113 bp	1.0	43.0
psbM-trnD-GAC_p5	AATGCAAGAATATTGACTTCCA	TCAATTGGTTAGAGCACCGC	31.8	50.0	54.0	58.6	1093-1115 bp	1.0	43.0