Hotspot

atpH-atpI

LSC · 13303-13804 · 502 bp

Back to Descurainia

Pi 0.0162

MarkerSeek score 67.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 8
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	664-669 bp	1.0	80.9
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	646-651 bp	1.0	80.9
atpH-atpI_p3	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	668-673 bp	1.0	80.3
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	641-646 bp	1.0	80.2
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	623-628 bp	1.0	80.1