primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	rps16-trnQ-CAA	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6034	6482	502-522	1.000000	83.4
rps16-trnQ-CAA_p2	rps16-trnQ-CAA	2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.308340	6034	6482	512-532	1.000000	82.6
rps16-trnQ-CAA_p3	rps16-trnQ-CAA	3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.491095	6034	6482	528-548	1.000000	81.0
rps16-trnQ-CAA_p4	rps16-trnQ-CAA	4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	0.0	16.9	0.499524	6034	6482	529-549	1.000000	80.9
rps16-trnQ-CAA_p5	rps16-trnQ-CAA	5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.4	58.1	0.0	16.9	0.710916	6034	6482	530-550	1.000000	79.2
trnG-GGA-trnR-AGA_p1	trnG-GGA-trnR-AGA	1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.105791	9215	9375	281-283	1.000000	49.1
trnG-GGA-trnR-AGA_p2	trnG-GGA-trnR-AGA	2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.109107	9215	9375	287-289	1.000000	49.1
trnG-GGA-trnR-AGA_p3	trnG-GGA-trnR-AGA	3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	57.9	53.9	33.4	3.0	4.132047	9215	9375	301-303	1.000000	48.8
trnG-GGA-trnR-AGA_p4	trnG-GGA-trnR-AGA	4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.1	53.9	0.0	3.0	4.151221	9215	9375	289-291	1.000000	48.7
trnG-GGA-trnR-AGA_p5	trnG-GGA-trnR-AGA	5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	20	21	50.0	38.1	58.0	54.8	0.0	3.0	4.230351	9215	9375	282-284	1.000000	48.1
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	3.3	0.536681	12591	13056	584-598	1.000000	76.8
atpF-atpH_p2	atpF-atpH	2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.5	57.6	0.0	3.3	0.955129	12591	13056	557-571	1.000000	73.5
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	20	20	50.0	45.0	57.9	57.1	0.0	3.3	1.007483	12591	13056	588-602	1.000000	73.0
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	20	20	50.0	55.0	57.9	59.0	0.0	0.0	1.093272	12591	13056	583-597	1.000000	72.3
atpF-atpH_p5	atpF-atpH	5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	20	20	55.0	50.0	58.8	57.6	0.0	3.3	1.173552	12591	13056	559-573	1.000000	71.7
atpH-atpI_p1	atpH-atpI	1	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.136455	13303	13804	664-669	1.000000	80.9
atpH-atpI_p2	atpH-atpI	2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.152946	13303	13804	646-651	1.000000	80.9
atpH-atpI_p3	atpH-atpI	3	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	20	20	55.0	50.0	58.2	58.0	0.0	5.7	0.214492	13303	13804	668-673	1.000000	80.3
atpH-atpI_p4	atpH-atpI	4	TACCTTGACCAACTCCAGGT	TTTTTGCCACTTTAGCTGCG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.245019	13303	13804	641-646	1.000000	80.2
atpH-atpI_p5	atpH-atpI	5	GTCCAATAGAAGCAAGCCCA	TTTTTGCCACTTTAGCTGCG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.261511	13303	13804	623-628	1.000000	80.1
rpoB-trnC-UGC_p1	rpoB-trnC-UGC	1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.227525	26132	27244	1201-1209	1.000000	80.2
rpoB-trnC-UGC_p2	rpoB-trnC-UGC	2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	57.9	58.4	0.0	14.6	0.500888	26132	27244	1183-1191	1.000000	78.0
rpoB-trnC-UGC_p3	rpoB-trnC-UGC	3	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.981431	26132	27244	1202-1210	1.000000	74.1
rpoB-trnC-UGC_p4	rpoB-trnC-UGC	4	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	20	19	55.0	52.6	57.9	58.0	0.0	0.0	1.152069	26132	27244	1223-1231	1.000000	72.7
rpoB-trnC-UGC_p5	rpoB-trnC-UGC	5	TCTTTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	59.1	58.1	0.0	0.0	1.163145	26132	27244	1195-1203	1.000000	72.7
psbM-trnD-GAC_p1	psbM-trnD-GAC	1	TGCAAGAATATTGACTTCCA	AATTGGTTAGAGCACCGCC	20	19	35.0	52.6	52.5	58.1	0.0	0.0	6.674132	28675	29703	1089-1111	1.000000	43.1
psbM-trnD-GAC_p2	psbM-trnD-GAC	2	TGCAAGAATATTGACTTCCA	TCAATTGGTTAGAGCACCGC	20	20	35.0	50.0	52.5	58.6	0.0	5.4	6.093400	28675	29703	1091-1113	1.000000	43.0
psbM-trnD-GAC_p3	psbM-trnD-GAC	3	ATGCAAGAATATTGACTTCCA	TCAATTGGTTAGAGCACCGC	21	20	33.3	50.0	53.2	58.6	0.0	5.4	6.381302	28675	29703	1092-1114	1.000000	43.0
psbM-trnD-GAC_p4	psbM-trnD-GAC	4	TGCAAGAATATTGACTTCCAT	TCAATTGGTTAGAGCACCGC	21	20	33.3	50.0	53.2	58.6	0.0	5.4	6.381302	28675	29703	1091-1113	1.000000	43.0
psbM-trnD-GAC_p5	psbM-trnD-GAC	5	AATGCAAGAATATTGACTTCCA	TCAATTGGTTAGAGCACCGC	22	20	31.8	50.0	54.0	58.6	0.0	5.4	6.507020	28675	29703	1093-1115	1.000000	43.0
trnD-GAC-trnY-UAC_p1	trnD-GAC-trnY-UAC	1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.789957	29778	30215	509-515	1.000000	75.9
trnD-GAC-trnY-UAC_p2	trnD-GAC-trnY-UAC	2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.791509	29778	30215	508-514	1.000000	75.9
trnD-GAC-trnY-UAC_p3	trnD-GAC-trnY-UAC	3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.6	58.4	7.1	0.0	0.902511	29778	30215	530-536	1.000000	74.9
trnD-GAC-trnY-UAC_p4	trnD-GAC-trnY-UAC	4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.6	57.6	7.1	0.0	0.926188	29778	30215	529-535	1.000000	74.7
trnD-GAC-trnY-UAC_p5	trnD-GAC-trnY-UAC	5	GCGGTGCTCTAACCAATTGA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.6	58.4	7.1	0.0	0.963468	29778	30215	563-569	1.000000	74.3
trnE-GAA-trnT-ACC_p1	trnE-GAA-trnT-ACC	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30432	31091	801-957	1.000000	80.1
trnE-GAA-trnT-ACC_p2	trnE-GAA-trnT-ACC	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	30432	31091	780-936	1.000000	79.7
trnE-GAA-trnT-ACC_p3	trnE-GAA-trnT-ACC	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	30432	31091	777-933	1.000000	79.5
trnE-GAA-trnT-ACC_p4	trnE-GAA-trnT-ACC	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30432	31091	763-919	1.000000	79.4
trnE-GAA-trnT-ACC_p5	trnE-GAA-trnT-ACC	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30432	31091	742-898	1.000000	79.3
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.125488	41494	42210	829-835	1.000000	80.3
psaA-ycf3_p2	psaA-ycf3	2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	20	20	50.0	50.0	57.9	58.0	6.3	0.0	0.134422	41494	42210	846-852	1.000000	80.2
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	20	20	50.0	45.0	57.9	57.7	4.1	0.0	0.369889	41494	42210	837-843	1.000000	78.4
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	41494	42210	891-897	1.000000	78.2
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.377504	41494	42210	893-899	1.000000	78.2
accD_p1	accD	1	CCATCCTGCATGTTGTCCTT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.2	57.9	0.0	26.8	0.260169	56576	57793	2233-2495	1.000000	80.7
accD_p2	accD	2	GGCTTGAACCAAGTATCCCC	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.2	57.9	8.3	26.8	0.339569	56576	57793	2264-2526	1.000000	80.1
accD_p3	accD	3	GGGCTTGAACCAAGTATCCC	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.2	57.9	8.3	26.8	0.339569	56576	57793	2265-2527	1.000000	80.1
accD_p4	accD	4	CCATCCTGCATGTTGTCCTT	CCGGAAAGACTAGGCCTACT	20	20	50.0	55.0	58.2	58.2	0.0	8.9	0.378691	56576	57793	2215-2477	1.000000	79.8
accD_p5	accD	5	CCATCCTGCATGTTGTCCTT	TGCAATTGCCGGAAAGACTA	20	20	50.0	45.0	58.2	57.8	0.0	26.9	0.349295	56576	57793	2223-2223	0.500000	57.2
rps11-rpl36_p1	rps11-rpl36	1	CGTGAACCACTACGTGTATT	ATCCGTAGAAGGGGACGAAT	20	20	45.0	50.0	55.5	57.9	0.0	0.0	2.537005	78145	78263	241-245	1.000000	61.0
rps11-rpl36_p2	rps11-rpl36	2	CGTGAACCACTACGTGTATT	AATCCGTAGAAGGGGACGAA	20	20	45.0	50.0	55.5	58.1	0.0	0.0	2.596748	78145	78263	242-246	1.000000	60.5
rps11-rpl36_p3	rps11-rpl36	3	CGTGAACCACTACGTGTATT	TCCGTAGAAGGGGACGAATT	20	20	45.0	50.0	55.5	58.1	0.0	0.0	2.596748	78145	78263	240-244	1.000000	60.5
rps11-rpl36_p4	rps11-rpl36	4	CGTGAACCACTACGTGTATT	GTTCCAACCCGAGGCATAAA	20	20	45.0	50.0	55.5	58.2	0.0	0.0	2.624398	78145	78263	210-214	1.000000	60.5
rps11-rpl36_p5	rps11-rpl36	5	CGTGAACCACTACGTGTATT	TTCCAACCCGAGGCATAAAC	20	20	45.0	50.0	55.5	58.2	0.0	0.0	2.624398	78145	78263	209-213	1.000000	60.5
rpl16-rps3_p1	rpl16-rps3	1	AACGAGTCACACACTAAGCA	AAAATTTGGATATTCGTAGACG	20	22	45.0	31.8	57.1	52.7	0.0	0.0	8.163676	81499	81672	225-229	1.000000	45.6
rpl16-rps3_p2	rpl16-rps3	2	AACGAGTCACACACTAAGCA	AAAAATTTGGATATTCGTAGACG	20	23	45.0	30.4	57.1	53.6	0.0	0.0	8.329662	81499	81672	226-230	1.000000	45.6
rpl16-rps3_p3	rpl16-rps3	3	ACCAACGAGTCACACACTAAG	AAAATTTGGATATTCGTAGACG	21	22	47.6	31.8	57.9	52.7	0.0	0.0	8.374466	81499	81672	228-232	1.000000	45.6
rpl16-rps3_p4	rpl16-rps3	4	AAACCAACGAGTCACACACT	AAAATTTGGATATTCGTAGACG	20	22	45.0	31.8	57.6	52.7	0.0	0.0	7.677338	81499	81672	230-234	1.000000	45.5
rpl16-rps3_p5	rpl16-rps3	5	AAACCAACGAGTCACACACT	AAAAATTTGGATATTCGTAGACG	20	23	45.0	30.4	57.6	53.6	0.0	0.0	7.843325	81499	81672	231-235	1.000000	45.5
ndhF-rpl32_p1	ndhF-rpl32	1	TCCATGAATATTGATATGTATGTTCCA	ACGATGTCCAATATCCCTTCC	27	21	29.6	47.6	56.7	57.2	0.0	0.0	10.024661	111416	112024	722-923	1.000000	43.8
ndhF-rpl32_p2	ndhF-rpl32	2	CCATGAATATTGATATGTATGTTCCA	ACGATGTCCAATATCCCTTCC	26	21	30.8	47.6	55.5	57.2	0.0	0.0	10.263220	111416	112024	721-922	1.000000	43.8
ndhF-rpl32_p3	ndhF-rpl32	3	TCCATGAATATTGATATGTATGTTCCA	CGATGTCCAATATCCCTTCCT	27	21	29.6	47.6	56.7	56.9	0.0	0.0	10.310777	111416	112024	721-922	1.000000	43.8
ndhF-rpl32_p4	ndhF-rpl32	4	TCCATGAATATTGATATGTATGTTCCA	AACGATGTCCAATATCCCTTCC	27	22	29.6	45.5	56.7	57.9	0.0	0.0	10.339790	111416	112024	723-924	1.000000	43.8
ndhF-rpl32_p5	ndhF-rpl32	5	TCCATGAATATTGATATGTATGTTCCA	AAGCTTTCAACGATGTCCAA	27	20	29.6	40.0	56.7	55.9	0.0	0.0	10.375075	111416	112024	731-932	1.000000	43.8
rpl32-trnL-CUA_p1	rpl32-trnL-CUA	1	GGGGACAATAAAAAGCACGC	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	58.0	58.0	0.0	6.7	0.023728	112184	113066	2142-2142	0.500000	59.8
rpl32-trnL-CUA_p2	rpl32-trnL-CUA	2	GGGGACAATAAAAAGCACGC	TCGAACCGAGATGCTTTAGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.086630	112184	113066	1632-1632	0.500000	59.3
rpl32-trnL-CUA_p3	rpl32-trnL-CUA	3	GGGGACAATAAAAAGCACGC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.133478	112184	113066	1606-1606	0.500000	58.9
rpl32-trnL-CUA_p4	rpl32-trnL-CUA	4	GGGGACAATAAAAAGCACGC	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.194205	112184	113066	1599-1599	0.500000	58.4
rpl32-trnL-CUA_p5	rpl32-trnL-CUA	5	GGGGACAATAAAAAGCACGC	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.205496	112184	113066	1604-1604	0.500000	58.4
rps15-ycf1_p1	rps15-ycf1	1	TCAACAGATCCCCTGCTTTC	ACACATGTATACGCGATTTCCA	20	22	50.0	40.9	57.5	58.2	0.0	15.9	2.709113	122331	122654	417-434	1.000000	61.1
rps15-ycf1_p2	rps15-ycf1	2	CAACAGATCCCCTGCTTTCT	ACACATGTATACGCGATTTCCA	20	22	50.0	40.9	57.5	58.2	0.0	15.9	2.716109	122331	122654	416-433	1.000000	61.1
rps15-ycf1_p3	rps15-ycf1	3	TCAACAGATCCCCTGCTTTC	CACATGTATACGCGATTTCCA	20	21	50.0	42.9	57.5	56.5	0.0	15.9	2.979880	122331	122654	416-433	1.000000	59.0
rps15-ycf1_p4	rps15-ycf1	4	TCAACAGATCCCCTGCTTTC	ACACATGTATACGCGATTTCC	20	21	50.0	42.9	57.5	56.5	0.0	15.9	2.982092	122331	122654	417-434	1.000000	59.0
rps15-ycf1_p5	rps15-ycf1	5	CAACAGATCCCCTGCTTTCT	CACATGTATACGCGATTTCCA	20	21	50.0	42.9	57.5	56.5	0.0	15.9	2.986876	122331	122654	415-432	1.000000	58.9
ycf1_p1	ycf1	1	TGGTATGATCCACGGTTTGA	ATTGAGCCCTGGGTTGATAC	20	20	45.0	50.0	56.5	57.3	0.0	15.6	2.191903	123761	123880	240-240	1.000000	65.8
ycf1_p2	ycf1	2	TGATTGGTATGATCCACGGT	ATTGAGCCCTGGGTTGATAC	20	20	45.0	50.0	56.3	57.3	0.0	15.6	2.425151	123761	123880	244-244	1.000000	63.9
ycf1_p3	ycf1	3	TTGGTATGATCCACGGTTTGA	ATTGAGCCCTGGGTTGATAC	21	20	42.9	50.0	57.3	57.3	0.0	15.6	2.441470	123761	123880	241-241	1.000000	63.8
ycf1_p4	ycf1	4	TGGTATGATCCACGGTTTGA	GCCCTGGGTTGATACCAAG	20	19	45.0	57.9	56.5	57.8	0.0	15.6	2.646415	123761	123880	235-235	1.000000	62.2
ycf1_p5	ycf1	5	TGGTATGATCCACGGTTTGA	AGCCCTGGGTTGATACCAA	20	19	45.0	52.6	56.5	58.2	0.0	15.6	2.655657	123761	123880	236-236	1.000000	62.1