Hotspot

petD

LSC · 72342-73561 · 1220 bp

Back to Deschampsia

Pi 0.00246

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1220
variable_sites: 3
pi: 0.00246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGATACTTCTTTTCAACTCCGA	CACGGTACTTCACGGTTGAA	39.1	50.0	57.5	58.1	1339 bp	1.0	51.2
petD_p2	ACTTCTTTTCAACTCCGAAGT	CACGGTACTTCACGGTTGAA	38.1	50.0	55.4	58.1	1334 bp	1.0	50.1
petD_p3	TGGATACTTCTTTTCAACTCCGA	CCTATGCACGGTACTTCACG	39.1	55.0	57.5	58.4	1345 bp	1.0	48.8
petD_p4	TGGATACTTCTTTTCAACTCCGA	CTATGCACGGTACTTCACGG	39.1	55.0	57.5	58.4	1344 bp	1.0	48.8
petD_p5	TGGATACTTCTTTTCAACTCCG	CACGGTACTTCACGGTTGAA	40.9	50.0	56.1	58.1	1339 bp	1.0	47.9