Hotspot

atpB

LSC · 51510-53006 · 1497 bp

Back to Deschampsia

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
LSC
length_bp
1497
variable_sites
2
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 CGTGGTTTGGTAATACGCCA TCTTTTGGATTTGATTTGGCA 50.0 33.3 58.6 54.5 1687 bp 1.0 40.1
atpB_p2 CGTGGTTTGGTAATACGCCA TTCTTTTGGATTTGATTTGGCA 50.0 31.8 58.6 55.3 1688 bp 1.0 40.1
atpB_p3 GGGGCGTGGTTTGGTAATAC TCTTTTGGATTTGATTTGGCA 55.0 33.3 58.9 54.5 1691 bp 1.0 40.1
atpB_p4 CGTGGTTTGGTAATACGCCA TCTTTTGGATTTGATTTGGCAT 50.0 31.8 58.6 55.1 1687 bp 1.0 40.1
atpB_p5 GGGGCGTGGTTTGGTAATAC TTCTTTTGGATTTGATTTGGCA 55.0 31.8 58.9 55.3 1692 bp 1.0 40.1