Hotspot

psaC-ndhE

SSC · 108523-109031 · 509 bp

Back to Deschampsia

Pi 0.00398

MarkerSeek score 60.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 509
variable_sites: 2
pi: 0.00398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	TTCTTTCTGCCATCCATCGT	55.0	45.0	58.0	57.2	678-684 bp	1.0	74.5
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	TTCTTTCTGCCATCCATCGT	55.0	45.0	58.0	57.2	679-685 bp	1.0	74.5
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	TTCTTTCTGCCATCCATCGT	50.0	45.0	58.1	57.2	639-645 bp	1.0	73.6
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	TTCTTTCTGCCATCCATCGT	50.0	45.0	58.1	57.2	640-646 bp	1.0	73.6
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	TTCTTTCTGCCATCCATCGT	50.0	45.0	57.9	57.2	641-647 bp	1.0	73.5