Hotspot

rpl32-trnL

SSC · 104715-105390 · 676 bp

Back to Deschampsia

Pi 0.00444

MarkerSeek score 61.30

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 676
variable_sites: 3
pi: 0.00444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	ATGAGCAGCATCCAAAACCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	795-854 bp	1.0	79.7
rpl32-trnL_p2	CATTTTCCAGCGGCAATGAG	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.1	58.0	810-869 bp	1.0	79.6
rpl32-trnL_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	775-834 bp	1.0	79.3
rpl32-trnL_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	774-833 bp	1.0	79.3
rpl32-trnL_p5	ATGAGCAGCATCCAAAACCA	GGATTCGAACCGAGATGCTT	45.0	50.0	58.1	58.1	798-857 bp	1.0	79.2