Hotspot

ndhF-rpl32

SSC · 103685-104531 · 847 bp

Back to Deschampsia

Pi 0.00708
MarkerSeek score 62.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
847
variable_sites
6
pi
0.00708
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGTGGGAGAAGGGGGATTAC GCGTGTACTATGGAAAAATACGT 55.0 39.1 58.1 57.3 986 bp 1.0 50.0
ndhF-rpl32_p2 AAGGCCAAATCCCATTGACA GCGTGTACTATGGAAAAATACGT 45.0 39.1 57.7 57.3 1017 bp 1.0 48.2
ndhF-rpl32_p3 AGTGGGAGAAGGGGGATTAC AGCGTGTACTATGGAAAAATACG 55.0 39.1 58.1 57.0 987 bp 1.0 48.0
ndhF-rpl32_p4 AGGCCAAATCCCATTGACAT GCGTGTACTATGGAAAAATACGT 45.0 39.1 57.4 57.3 1016 bp 1.0 46.4
ndhF-rpl32_p5 AAGGCCAAATCCCATTGACA AGCGTGTACTATGGAAAAATACG 45.0 39.1 57.7 57.0 1018 bp 1.0 46.2