Hotspot

atpB-rbcL

LSC · 53007-53858 · 852 bp

Back to Deschampsia

Pi 0.00126

MarkerSeek score 63.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 852
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AAAGTGACATCTAGCACGGG	CCTTGGTTTCATACTCCGGG	50.0	55.0	57.6	58.0	995-1053 bp	1.0	80.7
atpB-rbcL_p2	AAAGTGACATCTAGCACGGG	CTTGGTTTCATACTCCGGGG	50.0	55.0	57.6	58.0	994-1052 bp	1.0	80.7
atpB-rbcL_p3	AAAGTGACATCTAGCACGGG	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	57.6	58.2	983-1041 bp	1.0	79.7
atpB-rbcL_p4	AAAGTGACATCTAGCACGGG	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	57.6	58.2	982-1040 bp	1.0	79.7
atpB-rbcL_p5	AAAGTGACATCTAGCACGGG	TCCGGGGTGTAGTAAGTCAA	50.0	50.0	57.6	57.7	981-1039 bp	1.0	78.5