Hotspot

trnL-trnF

LSC · 46530-46880 · 351 bp

Back to Deschampsia

Pi 0.00285

MarkerSeek score 49.80

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 1
pi: 0.00285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	442 bp	1.0	75.5
trnL-trnF_p2	AAGTCGTGAGGGTTCAAGTC	CTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	57.8	457 bp	1.0	74.3
trnL-trnF_p3	AAGTCGTGAGGGTTCAAGTC	TCTGCCAGGAACCAGATTTG	50.0	50.0	57.5	57.8	458 bp	1.0	74.3
trnL-trnF_p4	AAGTCGTGAGGGTTCAAGTC	TTTTCTCTGCCAGGAACCAG	50.0	50.0	57.5	57.7	463 bp	1.0	73.7
trnL-trnF_p5	AAGTCGTGAGGGTTCAAGTC	TCTCTGCCAGGAACCAGATT	50.0	50.0	57.5	58.3	460 bp	1.0	73.2