Hotspot

trnT-UGU-trnL

LSC · 45141-45905 · 765 bp

Back to Deschampsia

Pi 0.00262

MarkerSeek score 60.40

Primer available yes

Label trnT-UGU-trnL

Feature Metadata

label_name: trnT-UGU-trnL
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 2
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL_p1	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	855-856 bp	1.0	78.1
trnT-UGU-trnL_p2	CGATGACCCTCGCATTACAA	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	847-848 bp	1.0	77.8
trnT-UGU-trnL_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	825-826 bp	1.0	77.2
trnT-UGU-trnL_p4	ATTTGAACCGATGACCCTCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.0	57.6	849-850 bp	1.0	76.8
trnT-UGU-trnL_p5	CGATGACCCTCGCATTACAA	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	841-842 bp	1.0	76.5