Hotspot

atpH-atpF

LSC · 31596-32063 · 468 bp

Back to Deschampsia

Pi 0.00649

MarkerSeek score 61.00

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 3
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCACTAGCGCTTTTATTTGC	CAGATGGCCAGTGAGCTAAA	45.0	50.0	56.4	57.6	547-553 bp	1.0	65.2
atpH-atpF_p2	GCACTAGCGCTTTTATTTGC	ATTAAGCCCGAAACTGCCAG	45.0	50.0	56.4	58.5	567-573 bp	1.0	64.1
atpH-atpF_p3	GCACTAGCGCTTTTATTTGC	TATTAAGCCCGAAACTGCCA	45.0	45.0	56.4	57.2	568-574 bp	1.0	62.1
atpH-atpF_p4	GCACTAGCGCTTTTATTTGC	TTAAGCCCGAAACTGCCAG	45.0	52.6	56.4	58.1	566-572 bp	1.0	59.9
atpH-atpF_p5	GCACTAGCGCTTTTATTTGC	GTATTAAGCCCGAAACTGCC	45.0	50.0	56.4	56.9	569-575 bp	1.0	59.4