Hotspot

trnC-rpoB

LSC · 18017-19034 · 1018 bp

Back to Deschampsia

Pi 0.00295

MarkerSeek score 60.40

Primer available yes

Label trnC-rpoB

Feature Metadata

label_name: trnC-rpoB
feature_type: igs
region: LSC
length_bp: 1018
variable_sites: 3
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1087-1187 bp	1.0	79.3
trnC-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1094-1194 bp	1.0	78.2
trnC-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	50.0	50.0	58.1	57.7	1170-1270 bp	1.0	77.5
trnC-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1102-1202 bp	1.0	77.0
trnC-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1101-1201 bp	1.0	77.0