Hotspot

petN-trnC

LSC · 17111-17945 · 835 bp

Back to Deschampsia

Pi 0.0024

MarkerSeek score 60.20

Primer available yes

Label petN-trnC

Feature Metadata

label_name: petN-trnC
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 2
pi: 0.0024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC_p1	AGTCCACTCCTTCCCCATAC	TTTCTCGATTTTCGCTCGGA	55.0	45.0	58.1	57.6	1018 bp	1.0	76.4
petN-trnC_p2	GTCCACTCCTTCCCCATACT	TTTCTCGATTTTCGCTCGGA	55.0	45.0	58.1	57.6	1017 bp	1.0	76.4
petN-trnC_p3	AGCCCAAGCGAGACTTACTA	TTTCTCGATTTTCGCTCGGA	50.0	45.0	58.2	57.6	959 bp	1.0	76.2
petN-trnC_p4	GAGTCCACTCCTTCCCCATA	TTTCTCGATTTTCGCTCGGA	55.0	45.0	57.8	57.6	1019 bp	1.0	76.0
petN-trnC_p5	CTAGAGTCCACTCCTTCCCC	TTTCTCGATTTTCGCTCGGA	60.0	45.0	58.2	57.6	1022 bp	1.0	75.6