Hotspot

trnY-trnD

LSC · 15565-15876 · 312 bp

Back to Deschampsia

Pi 0.00641

MarkerSeek score 56.40

Primer available yes

Label trnY-trnD

Feature Metadata

label_name: trnY-trnD
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 2
pi: 0.00641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-trnD_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	436 bp	1.0	76.2
trnY-trnD_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	403 bp	1.0	73.8
trnY-trnD_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	402 bp	1.0	73.6
trnY-trnD_p4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	435 bp	1.0	72.0
trnY-trnD_p5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	404 bp	1.0	70.4