Hotspot

ycf1

SSC · 123127-128502 · 5376 bp

Back to Derris

Pi 0.0177
MarkerSeek score 48.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5376
variable_sites
95
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AAGTCCCCTTTTCTTTTCTT TCAACAATTGACAAACATAATCCA 35.0 29.2 52.4 55.5 174 bp 1.0 42.8
ycf1_p2 AGTCCCCTTTTCTTTTCTTT TCAACAATTGACAAACATAATCCA 35.0 29.2 52.4 55.5 173 bp 1.0 42.8
ycf1_p3 AAGTCCCCTTTTCTTTTCTTT TCAACAATTGACAAACATAATCCA 33.3 29.2 53.4 55.5 174 bp 1.0 42.8
ycf1_p4 AGTCCCCTTTTCTTTTCTTTT TCAACAATTGACAAACATAATCCA 33.3 29.2 53.4 55.5 173 bp 1.0 42.8
ycf1_p5 AAAGTCCCCTTTTCTTTTCT TCAACAATTGACAAACATAATCCA 35.0 29.2 52.4 55.5 175 bp 1.0 42.7