Hotspot

ndhG-ndhI

SSC · 117781-118220 · 440 bp

Back to Derris

Pi 0.0393
MarkerSeek score 64.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
440
variable_sites
17
pi
0.0393
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 GAGCCACGATAATTGCACCT AATCAAATTGCTTTGGGCCG 50.0 45.0 58.3 57.9 1050-1051 bp 1.0 78.9
ndhG-ndhI_p2 GAGCCACGATAATTGCACCT TGGTCAACAAACAGTCCGAG 50.0 50.0 58.3 58.1 1429-1430 bp 1.0 78.7
ndhG-ndhI_p3 GAGCCACGATAATTGCACCT CTGCGAGGTACATTGGTCAA 50.0 50.0 58.3 57.9 1409-1410 bp 1.0 78.4
ndhG-ndhI_p4 CCATCCCCAACAGTCCAAAG TGGTCAACAAACAGTCCGAG 55.0 50.0 58.5 58.1 1198-1199 bp 1.0 78.0
ndhG-ndhI_p5 ATCGGTTCAGGCAAATCCAT TGGTCAACAAACAGTCCGAG 45.0 50.0 57.6 58.1 926 bp 0.5 56.0