Hotspot

trnR-ACG-trnN-GUU

IRb · 108079-108716 · 638 bp

Back to Derris

Pi 0.0173
MarkerSeek score 68.80
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
638
variable_sites
11
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 779 bp 1.0 79.5
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.8 58.1 820 bp 1.0 78.7
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 55.0 50.0 57.8 57.8 743 bp 1.0 78.4
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 730 bp 1.0 78.3
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 55.0 50.0 57.8 57.8 819 bp 1.0 77.9