Hotspot

rps3-rps19

LSC · 83379-83823 · 445 bp

Back to Derris

Pi 0.0256
MarkerSeek score 60.40
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
445
variable_sites
11
pi
0.0256
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 CAAGTCTGAAACCAAGCGGA AAATGATAATAGATCTCGTCGT 50.0 31.8 58.4 52.7 503-507 bp 1.0 44.1
rps3-rps19_p2 CAAGTCTGAAACCAAGCGGA AATGATAATAGATCTCGTCGTT 50.0 31.8 58.4 52.7 502-506 bp 1.0 44.1
rps3-rps19_p3 CAAGTCTGAAACCAAGCGGA AAAATGATAATAGATCTCGTCGT 50.0 30.4 58.4 53.5 504-508 bp 1.0 44.1
rps3-rps19_p4 CAAGTCTGAAACCAAGCGGA AAATGATAATAGATCTCGTCGTT 50.0 30.4 58.4 53.5 503-507 bp 1.0 44.1
rps3-rps19_p5 AGTCTGAAACCAAGCGGATT AATGATAATAGATCTCGTCGTT 45.0 31.8 57.4 52.7 500-504 bp 1.0 44.1