Hotspot

rpl36-rps8

LSC · 79492-79997 · 506 bp

Back to Derris

Pi 0.0304
MarkerSeek score 63.30
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
506
variable_sites
15
pi
0.0304
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 AACAGAAGCTCCTATTTTCA TCGGCGGAGAAGTTTTATGT 35.0 45.0 52.1 57.2 559-562 bp 1.0 44.0
rpl36-rps8_p2 AACAGAAGCTCCTATTTTCAT TCGGCGGAGAAGTTTTATGT 33.3 45.0 52.8 57.2 559-562 bp 1.0 44.0
rpl36-rps8_p3 AACAGAAGCTCCTATTTTCA TCGGCGGAGAAGTTTTATGTT 35.0 42.9 52.1 58.0 559-562 bp 1.0 44.0
rpl36-rps8_p4 AACAGAAGCTCCTATTTTCA ATCGGCGGAGAAGTTTTATGT 35.0 42.9 52.1 57.7 560-563 bp 1.0 44.0
rpl36-rps8_p5 AACAGAAGCTCCTATTTTCAT TCGGCGGAGAAGTTTTATGTT 33.3 42.9 52.8 58.0 559-562 bp 1.0 44.0