Hotspot

rps11-rpl36

LSC · 79050-79377 · 328 bp

Back to Derris

Pi 0.0411
MarkerSeek score 70.00
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
328
variable_sites
13
pi
0.0411
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 TCCTTTACGCGAACCCATTT GCAGACGAGGGCGAATTATA 45.0 50.0 57.8 57.6 417-429 bp 1.0 79.8
rps11-rpl36_p2 ACGCGAACCCATTTTTGGTA GCAGACGAGGGCGAATTATA 45.0 50.0 58.4 57.6 411-423 bp 1.0 78.2
rps11-rpl36_p3 TACGCGAACCCATTTTTGGT GCAGACGAGGGCGAATTATA 45.0 50.0 58.4 57.6 412-424 bp 1.0 78.2
rps11-rpl36_p4 TTACGCGAACCAATACGTCC GCAGACGAGGGCGAATTATA 50.0 50.0 58.4 57.6 434-446 bp 1.0 78.1
rps11-rpl36_p5 GTCCTTTACGCGAACCCATT GCAGACGAGGGCGAATTATA 50.0 50.0 58.6 57.6 418-430 bp 1.0 76.8