Hotspot

petD-rpoA

LSC · 77370-77553 · 184 bp

Back to Derris

Pi 0.0761
MarkerSeek score 70.90
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
184
variable_sites
14
pi
0.0761
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 CATTTCGTCGTCCAGTAGCA GAAGCCTCCCGGAATTTGAT 50.0 50.0 58.0 57.9 654-703 bp 1.0 82.2
petD-rpoA_p2 AAAACCCATTTCGTCGTCCA GAAGCCTCCCGGAATTTGAT 45.0 50.0 58.0 57.9 660-709 bp 1.0 82.0
petD-rpoA_p3 CATTTCGTCGTCCAGTAGCA CAAGGAAGCGCTTTGTGAAG 50.0 50.0 58.0 58.0 670 bp 0.5 59.6
petD-rpoA_p4 AAAACCCATTTCGTCGTCCA CAAGGAAGCGCTTTGTGAAG 45.0 50.0 58.0 58.0 676 bp 0.5 59.4
petD-rpoA_p5 GTACCATAGTGGCCCTTTGG CAAGGAAGCGCTTTGTGAAG 55.0 50.0 57.9 58.0 631 bp 0.5 59.2