Hotspot

clpP-psbB

LSC · 71565-72002 · 438 bp

Back to Derris

Pi 0.0365
MarkerSeek score 68.40
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
438
variable_sites
16
pi
0.0365
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CAACCCAAGATGCATCTCCA AAACGGCCCGGATCATTTAA 50.0 45.0 57.9 57.9 558-560 bp 1.0 80.2
clpP-psbB_p2 CTTTTGGAACACCAATGGGC AAACGGCCCGGATCATTTAA 50.0 45.0 57.8 57.9 516-518 bp 1.0 80.1
clpP-psbB_p3 TTTGGAACACCAATGGGCAT AAACGGCCCGGATCATTTAA 45.0 45.0 58.3 57.9 514-516 bp 1.0 79.2
clpP-psbB_p4 CAACCCAAGATGCATCTCCA AACGGCCCGGATCATTTAAT 50.0 45.0 57.9 57.6 557-559 bp 1.0 78.4
clpP-psbB_p5 CTTTTGGAACACCAATGGGC AACGGCCCGGATCATTTAAT 50.0 45.0 57.8 57.6 515-517 bp 1.0 78.3