Hotspot

psaJ-rpl33

LSC · 66581-67056 · 476 bp

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Pi 0.0449
MarkerSeek score 74.40
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
476
variable_sites
21
pi
0.0449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTGGCGGGTCTCTTGATAGA CTGATATCTTTACCCTTCGCCA 50.0 45.5 58.1 57.7 571-593 bp 1.0 64.2
psaJ-rpl33_p2 TTGGCGGGTCTCTTGATAGA TGATATCTTTACCCTTCGCCA 50.0 42.9 58.1 56.5 570-592 bp 1.0 63.0
psaJ-rpl33_p3 TTGGCGGGTCTCTTGATAGA TCTGATATCTTTACCCTTCGCC 50.0 45.5 58.1 57.4 572-594 bp 1.0 62.0
psaJ-rpl33_p4 TTGGCGGGTCTCTTGATAGA TGATCACAACTGGTACATTCCA 50.0 40.9 58.1 57.4 607-629 bp 1.0 61.6
psaJ-rpl33_p5 TTTGGCGGGTCTCTTGATAG CTGATATCTTTACCCTTCGCCA 50.0 45.5 57.3 57.7 572-594 bp 1.0 59.8