Hotspot

trnW-CCA-trnP-UGG

LSC · 65983-66005 · 23 bp

Back to Derris

Pi 0.087
MarkerSeek score 78.40
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
23
variable_sites
2
pi
0.087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 90-208 bp 1.0 79.8
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 91-209 bp 1.0 79.8
trnW-CCA-trnP-UGG_p3 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 80-198 bp 1.0 79.8
trnW-CCA-trnP-UGG_p4 GCGTTCTACCGAACTGAACT CTTGGTAGCGCGTTTGTTTT 50.0 45.0 57.9 57.9 110-228 bp 1.0 79.5
trnW-CCA-trnP-UGG_p5 GCGTTCTACCGAACTGAACT CCGATGGAAAGGGATGTAGC 50.0 55.0 57.9 58.1 134-252 bp 1.0 79.4