Hotspot

atpH-atpF

LSC · 47949-48514 · 566 bp

Back to Derris

Pi 0.0338
MarkerSeek score 68.00
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
566
variable_sites
19
pi
0.0338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 GGCTGGTTGTAGCATTAGCA AGCCCAAGCAAAGGAAAGAA 50.0 45.0 58.2 57.9 650-669 bp 1.0 80.1
atpH-atpF_p2 GGCTGGTTGTAGCATTAGCA CAGTAGCCCAAGCAAAGGAA 50.0 50.0 58.2 58.1 654-673 bp 1.0 80.1
atpH-atpF_p3 TGGGCTGGTTGTAGCATTAG AGCCCAAGCAAAGGAAAGAA 50.0 45.0 57.6 57.9 652-671 bp 1.0 78.7
atpH-atpF_p4 TGGGCTGGTTGTAGCATTAG CAGTAGCCCAAGCAAAGGAA 50.0 50.0 57.6 58.1 656-675 bp 1.0 78.7
atpH-atpF_p5 AGGCAGAGGGAAAAATACGG AGCCCAAGCAAAGGAAAGAA 50.0 45.0 57.6 57.9 719-738 bp 1.0 78.5