Hotspot

rpoC2-rps2

LSC · 44733-44966 · 234 bp

Back to Derris

Pi 0.0812
MarkerSeek score 73.50
Primer available yes
Label rpoC2-rps2

Feature Metadata

label_name
rpoC2-rps2
feature_type
igs
region
LSC
length_bp
234
variable_sites
19
pi
0.0812
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rps2_p1 TGTGATACATCAGAACAATATAGGGT CCTGCTTCCATCATCTCTTCC 34.6 52.4 57.7 58.2 314-318 bp 1.0 44.8
rpoC2-rps2_p2 TGTGATACATCAGAACAATATAGGGT ACTCCTGCTTCCATCATCTCT 34.6 47.6 57.7 58.2 317-321 bp 1.0 44.8
rpoC2-rps2_p3 TGTGATACATCAGAACAATATAGGGT TGAACTCCTGCTTCCATCATC 34.6 47.6 57.7 57.7 320-324 bp 1.0 44.7
rpoC2-rps2_p4 TGTGATACATCAGAACAATATAGGGT CCGTGACCAAAATGAACTCC 34.6 50.0 57.7 57.0 332-336 bp 1.0 44.6
rpoC2-rps2_p5 TGTGATACATCAGAACAATATAGGGT TGACCAAAATGAACTCCTGCT 34.6 42.9 57.7 57.8 329-333 bp 1.0 44.6