Hotspot

ccsA-ndhD

Genome · 111746-112163 · 418 bp

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Pi 0.0758

MarkerSeek score 80.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 418
variable_sites: 55
pi: 0.0758
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTATGCTGACTCACGCCTTT	AACGGAGCCATATCGCAAAT	50.0	45.0	58.0	58.0	1554-1569 bp	1.0	86.9
ccsA-ndhD_p2	CTATGCTGACTCACGCCTTT	AGTTACTGATTCACGCGCAT	50.0	45.0	58.0	58.0	1739-1754 bp	1.0	86.7
ccsA-ndhD_p3	CTATGCTGACTCACGCCTTT	TGGAGTTACTGATTCACGCG	50.0	50.0	58.0	58.0	1742-1757 bp	1.0	86.6
ccsA-ndhD_p4	GGTTTGCTTCTTGGGAGTCA	TGGAGTTACTGATTCACGCG	50.0	50.0	58.0	58.0	1177-1192 bp	0.75	76.0
ccsA-ndhD_p5	GGTTTGCTTCTTGGGAGTCA	AGTTACTGATTCACGCGCAT	50.0	45.0	58.0	58.0	1174-1189 bp	0.75	76.0