Hotspot

petG-trnW-CCA

Genome · 62533-62792 · 260 bp

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Pi 0.0724

MarkerSeek score 75.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 260
variable_sites: 34
pi: 0.0724
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GCGATCAGCTAGATATTCGGT	CAAAACCCGATGTCGTAGGT	47.6	50.0	57.5	57.9	321-322 bp	1.0	75.6
petG-trnW-CCA_p2	GCGATCAGCTAGATATTCGGT	CTCCAAAACCCGATGTCGTA	47.6	50.0	57.5	57.6	324-325 bp	1.0	73.3
petG-trnW-CCA_p3	GCGATCAGCTAGATATTCGGT	TCCAAAACCCGATGTCGTAG	47.6	50.0	57.5	57.6	323-324 bp	1.0	73.3
petG-trnW-CCA_p4	GCGATCAGCTAGATATTCGGT	AAACCCGATGTCGTAGGTTC	47.6	50.0	57.5	57.6	319-320 bp	1.0	73.3
petG-trnW-CCA_p5	GCGATCAGCTAGATATTCGGT	CCGATGTCGTAGGTTCGAAT	47.6	50.0	57.5	57.5	315-316 bp	1.0	72.4