Hotspot

petA-psbJ

Genome · 59767-60611 · 845 bp

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Pi 0.0521

MarkerSeek score 81.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 845
variable_sites: 79
pi: 0.0521
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTTGCGTATTCAGGGTCTCA	GGGTCGTCTCCCCAATAA	50.0	55.6	57.6	55.5	977-998 bp	1.0	48.7
petA-psbJ_p2	AGTCCAGTTGGCAGAAATGA	GGGTCGTCTCCCCAATAA	45.0	55.6	57.0	55.5	890-911 bp	1.0	48.2
petA-psbJ_p3	CGGTTGTCTTGGCACAGATT	GGGTCGTCTCCCCAATAA	50.0	55.6	58.8	55.5	942-963 bp	1.0	47.7
petA-psbJ_p4	TTTTTCGCGTCGGTTGTCTT	GGGTCGTCTCCCCAATAA	45.0	55.6	59.0	55.5	952-973 bp	1.0	47.7
petA-psbJ_p5	CGTTGCGTATTCAGGGTCTC	GGGTCGTCTCCCCAATAA	55.0	55.6	58.7	55.5	978-999 bp	1.0	47.4