Hotspot

ndhC-trnV-UAC

Genome · 48390-48877 · 488 bp

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Pi 0.0502
MarkerSeek score 77.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
488
variable_sites
46
pi
0.0502
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTTGACACACCCCATTCAGG AAAGGAGGTTGGTGCATCAG 50.0 50.0 58.0 58.1 1128-1136 bp 1.0 85.2
ndhC-trnV-UAC_p2 CCAGCGGTAACAACTAGGTC AAAGGAGGTTGGTGCATCAG 55.0 50.0 58.0 58.1 1320-1328 bp 1.0 84.5
ndhC-trnV-UAC_p3 CGGTAACAACTAGGTCTGCC AAAGGAGGTTGGTGCATCAG 55.0 50.0 58.0 58.1 1316-1324 bp 1.0 84.5
ndhC-trnV-UAC_p4 CCAGCGGTAACAACTAGGTC GAGCCCTATGCCATAGAAGC 55.0 55.0 58.0 57.9 1255-1263 bp 0.75 74.1
ndhC-trnV-UAC_p5 CGGTAACAACTAGGTCTGCC GAGCCCTATGCCATAGAAGC 55.0 55.0 58.0 57.9 1251-1259 bp 0.75 74.1