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rps4-trnL-UAA

Genome · 44786-45453 · 668 bp

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Pi 0.0452

MarkerSeek score 76.70

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 668
variable_sites: 56
pi: 0.0452
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	GAGGTCCTCGGTAGCGAG	TCCATACCCAGGCTCAATCT	66.7	50.0	58.6	57.8	747-766 bp	1.0	63.9
rps4-trnL-UAA_p2	GAGGTCCTCGGTAGCGAG	TCTCCATACCCAGGCTCAAT	66.7	50.0	58.6	57.8	749-768 bp	1.0	63.8
rps4-trnL-UAA_p3	GAGGTCCTCGGTAGCGAG	CCATACCCAGGCTCAATCTG	66.7	55.0	58.6	57.7	746-765 bp	1.0	63.3
rps4-trnL-UAA_p4	GAGGTCCTCGGTAGCGAG	CTACCATTCCGCCATATCCC	66.7	55.0	58.6	57.6	710-729 bp	1.0	62.5
rps4-trnL-UAA_p5	GGTCCTCGGTAGCGAGAC	TCCATACCCAGGCTCAATCT	66.7	50.0	58.9	57.8	745-764 bp	1.0	61.4