Hotspot

psbD-trnT-GGU

Genome · 33980-35390 · 1411 bp

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Pi 0.0513

MarkerSeek score 83.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1411
variable_sites: 134
pi: 0.0513
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TCTTCTGAGCCAGTCATCCA	TTCAAATCCGACAAAGGGCT	50.0	45.0	58.1	57.7	1474-1516 bp	1.0	86.9
psbD-trnT-GGU_p2	TCTTCTGAGCCAGTCATCCA	GTTCAAATCCGACAAAGGGC	50.0	50.0	58.1	57.7	1475-1517 bp	1.0	86.5
psbD-trnT-GGU_p3	CCACGAAGACAAAGCGATCT	TTCAAATCCGACAAAGGGCT	50.0	45.0	58.3	57.7	1493-1535 bp	1.0	85.0
psbD-trnT-GGU_p4	AAAGCGATCTCTTCTGAGCC	TTCAAATCCGACAAAGGGCT	50.0	45.0	57.7	57.7	1483-1525 bp	1.0	84.9
psbD-trnT-GGU_p5	ACCAACCCACGAAGACAAAG	TTCAAATCCGACAAAGGGCT	50.0	45.0	58.3	57.7	1499-1541 bp	1.0	84.7