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psbM-petN

Genome · 28256-29133 · 878 bp

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Pi 0.0565

MarkerSeek score 83.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 878
variable_sites: 90
pi: 0.0565
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCCACCGCACTTTTTATTCC	TCACGCAGAAACAATCCCAA	50.0	45.0	58.0	58.0	1346-1373 bp	1.0	89.1
psbM-petN_p2	GCCACCGCACTTTTTATTCC	ATTGATAACCCGAACGTCCG	50.0	50.0	58.0	58.1	1311-1338 bp	1.0	89.0
psbM-petN_p3	GCCACCGCACTTTTTATTCC	TTTCATGTGTCTCGATGCGT	50.0	45.0	58.0	57.9	1365-1392 bp	1.0	88.6
psbM-petN_p4	GCCACCGCACTTTTTATTCC	CCTCAAGCTTTGCCTCGTAT	50.0	50.0	58.0	58.0	1122-1130 bp	0.75	75.4
psbM-petN_p5	GCCACCGCACTTTTTATTCC	GTCGCCTAACGGGAAAATCT	50.0	50.0	58.0	58.0	1544-1552 bp	0.5	61.1