Hotspot

rpoB-trnD-GUC

Genome · 26524-27281 · 758 bp

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Pi 0.0418

MarkerSeek score 79.40

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 758
variable_sites: 58
pi: 0.0418
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.0	58.6	803-811 bp	1.0	82.5
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.0	58.6	804-812 bp	1.0	82.5
rpoB-trnD-GUC_p3	AGTTCTGGTAGTACGGGCAT	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.1	58.6	801-809 bp	1.0	81.4
rpoB-trnD-GUC_p4	AAGTTCTGGTAGTACGGGCA	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.4	58.6	802-810 bp	1.0	79.6
rpoB-trnD-GUC_p5	CAAGTTCTGGTAGTACGGGC	GGATTGACGGGTCTCGAAC	55.0	57.9	58.0	58.0	844-852 bp	1.0	78.2