Hotspot

atpH-atpI

Genome · 13538-14283 · 746 bp

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Pi 0.0481

MarkerSeek score 76.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 746
variable_sites: 65
pi: 0.0481
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GTCAAAGGAAAATCGCGGTG	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.0	58.0	1397-1419 bp	0.75	73.7
atpH-atpI_p2	TCGAAAACAGTTTGGGGGAG	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.0	58.0	1202 bp	0.5	60.4
atpH-atpI_p3	TCGAAAACAGTTTGGGGGAG	GGCGAATCGTTCAGTTACCT	50.0	50.0	58.0	58.0	1446 bp	0.5	60.3
atpH-atpI_p4	TCGAAAACAGTTTGGGGGAG	TACCTATCGTAGCCACCAGG	50.0	55.0	58.0	58.0	1641 bp	0.5	60.3
atpH-atpI_p5	TCGAAAACAGTTTGGGGGAG	ATTACGCACATCGAGCAACT	50.0	45.0	58.0	58.0	1801 bp	0.5	60.3