Hotspot

ycf12-trnG-UCC

Genome · 8622-8794 · 173 bp

Back to Deparia

Pi 0.0614
MarkerSeek score 77.60
Primer available yes
Label ycf12-trnG-UCC

Feature Metadata

label_name
ycf12-trnG-UCC
feature_type
igs
region
Genome
length_bp
173
variable_sites
19
pi
0.0614
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf12-trnG-UCC_p1 ATGGAGCCAAATTCCGGATC TCTCTCCTCTTTCCACCGTT 50.0 50.0 58.0 58.0 1352-1370 bp 0.75 75.5
ycf12-trnG-UCC_p2 ATGGAGCCAAATTCCGGATC TTCTCTCCTCTTTCCACCGT 50.0 50.0 58.0 58.0 1353-1371 bp 0.75 75.5
ycf12-trnG-UCC_p3 GATGGAGCCAAATTCCGGAT TCTCTCCTCTTTCCACCGTT 50.0 50.0 58.0 58.0 1353-1371 bp 0.75 75.5
ycf12-trnG-UCC_p4 GATGGAGCCAAATTCCGGAT TTCTCTCCTCTTTCCACCGT 50.0 50.0 58.0 58.0 1354-1372 bp 0.75 75.5
ycf12-trnG-UCC_p5 GAGCCAAATTCCGGATCACT TCTCTCCTCTTTCCACCGTT 50.0 50.0 58.0 58.0 1349-1367 bp 0.75 75.3