Hotspot

trnS-GCU-ycf12

Genome · 7960-8519 · 560 bp

Back to Deparia

Pi 0.0601

MarkerSeek score 82.40

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: Genome
length_bp: 560
variable_sites: 62
pi: 0.0601
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	CAGGTTCATCGATCCACCAG	ACGCCAAAATAGCAAGTTGC	55.0	45.0	58.1	57.9	712-736 bp	1.0	88.3
trnS-GCU-ycf12_p2	TTTGTCGAATGATCCCGGAC	ACGCCAAAATAGCAAGTTGC	50.0	45.0	58.0	57.9	788-817 bp	1.0	87.8
trnS-GCU-ycf12_p3	GACAGGTTCATCGATCCACC	ACGCCAAAATAGCAAGTTGC	55.0	45.0	58.1	57.9	714-738 bp	0.75	77.9
trnS-GCU-ycf12_p4	GAGGGGAGAGAGAGGGATTC	ACGCCAAAATAGCAAGTTGC	60.0	45.0	58.0	57.9	675-691 bp	0.75	76.1
trnS-GCU-ycf12_p5	CCTACACCCACGGCTAGATA	ACGCCAAAATAGCAAGTTGC	55.0	45.0	58.0	57.9	1226-1254 bp	0.75	73.8