Hotspot

ycf1

SSC · 107522-112600 · 5079 bp

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Pi 0.000895

MarkerSeek score 49.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5079
variable_sites: 16
pi: 0.000895
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTTCGATGCAACAGCAAG	GATTTCCTTGAGACGGTCCC	45.0	55.0	57.6	58.0	1494 bp	1.0	77.0
ycf1_p2	TGTTTCGATGCAACAGCAAG	GGATTTCCTTGAGACGGTCC	45.0	55.0	57.6	58.0	1495 bp	1.0	77.0
ycf1_p3	TGTTTCGATGCAACAGCAAG	GGGATTTCCTTGAGACGGTC	45.0	55.0	57.6	58.0	1496 bp	1.0	77.0
ycf1_p4	TGTTTCGATGCAACAGCAAG	GAGACGGTCCCCAAATAACC	45.0	55.0	57.6	58.0	1485 bp	1.0	77.0
ycf1_p5	TGTTTCGATGCAACAGCAAG	GTGATCGAGCCCTTGCATTA	45.0	50.0	57.6	58.0	1271 bp	1.0	77.0