Hotspot

rpl32-ndhF

SSC · 122548-123607 · 1060 bp

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Pi 0.00226

MarkerSeek score 55.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1060
variable_sites: 11
pi: 0.00226
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1246-1257 bp	1.0	80.4
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1235-1246 bp	1.0	80.3
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1244-1255 bp	1.0	80.3
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	1245-1256 bp	1.0	80.3
rpl32-ndhF_p5	AACGCTGCCCAATATCCTTT	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1233-1244 bp	1.0	80.2