Hotspot

ndhI-ndhG

SSC · 117122-117482 · 361 bp

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Pi 0.00891

MarkerSeek score 55.20

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 9
pi: 0.00891
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TCAAATTGCTTTGGGTCGTT	GACCCAATCCCAGAAAGACT	40.0	50.0	56.4	56.8	497-504 bp	1.0	61.8
ndhI-ndhG_p2	TCAAATTGCTTTGGGTCGTT	AGACCCAATCCCAGAAAGAC	40.0	50.0	56.4	56.8	498-505 bp	1.0	61.7
ndhI-ndhG_p3	TCAAATTGCTTTGGGTCGTT	AGACCCAATCCCAGAAAGACT	40.0	47.6	56.4	58.4	498-505 bp	1.0	60.3
ndhI-ndhG_p4	ATCAAATTGCTTTGGGTCGT	AGACCCAATCCCAGAAAGAC	40.0	50.0	56.2	56.8	499-506 bp	1.0	59.9
ndhI-ndhG_p5	ATCAAATTGCTTTGGGTCGT	GACCCAATCCCAGAAAGACT	40.0	50.0	56.2	56.8	498-505 bp	1.0	59.9