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psbE-petL

LSC · 63873-65105 · 1233 bp

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Pi 0.00209

MarkerSeek score 45.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1233
variable_sites: 6
pi: 0.00209
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1424-1427 bp	1.0	72.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1347-1350 bp	1.0	71.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1433-1436 bp	1.0	71.5
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1438-1441 bp	1.0	71.4
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1432-1435 bp	1.0	71.4