Hotspot

petA-psbJ

LSC · 62329-63082 · 754 bp

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Pi 0.000482

MarkerSeek score 44.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 2
pi: 0.000482
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	892-912 bp	1.0	76.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	882-902 bp	1.0	76.8
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	930-950 bp	1.0	75.2
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	929-949 bp	1.0	75.2
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	57.9	57.4	920-940 bp	1.0	75.2